... It was held under the sponsorship of the Czech Immunological Society
and the Czech Society for Analytical Cytology. Workshop offers good
opportunity for making contacts and discussions about questions
interesting for veterinary immunologists, it means those, for which
laboratory mouse is not the dominant subject of their studies.
participants from various institutions took part in this-year seminar,
especially from the Veterinary Research Institute, Brno; University of
Veterinary and Pharmaceutical Sciences, Brno; Academy of Sciences
(Institute of Microbiology, Institute of Animal Physiology and Genetics,
Institute of Molecular Genetics).
The whole seminar was composed as a presentation of methodical
approaches used while solving different projects. The opening brief
presentation was rather a discussion topic on advantages or limitations
of individual models than presentation of results. Scientific programme
was divided into five sections.
In the first section – methods of molecular biology in immunology,
new methodical approaches were presented in characterization of the
immune response, gene rearrangement or intestinal microflora detection.
Genomics, detection of VDJ rearrangement or sequencing of ribosomal RNA
for characterization of microorganisms represent new trends in the
methodology of immunology.
The second section – mucosal immunity – was, after an introduction on
common system of mucosae and importance of microflora for activation of
the immune system, oriented to two organs in whose protection mucosal
immunity plays an important role – respiratory tract (pig and horse) and
mammary gland (cattle). Methods of sample collection from this organs
were described; the method of bronchoalveolar lavages, nasal and mammary
gland lavages. The possibilities of cell population and subpopulation
determination in those samples were shown using flow cytometry, and the
ways of further processing of samples were discussesd for objective
quantification of immunoglobulines or other soluble agents.
The third section was focused on methodical approaches in basic
research in veterinary immunology. New knowledge obtained on postnatal
functional maturation of the immune system of pigs and in the study of
development of blood elements in pigs and dogs was presented there.
On the second day of the seminar two sections were running. Clinical
immunology attracted attention of especially veterinary practitioners.
Besides the general introductory papers, further contributions were
devoted to individual nosological entities and possibilities of their
diagnosis – immunity-mediated hemolytic anemia, systemic lupus
erythematosus, rheumatoid arthritis. The possibilities of
allerodiagnosis in small animals were also mentioned as well as the
advantages and disadvantages of individual diagnostic methods and their
asset in proper diagnosis. The question concerning the importance of
secondary immunodeficiency in large production units of farm animals
remained unanswered. In this field experience obtained so far in the
Czech Republic is insufficient.
The last section was the most controversial,
discussing the experience with “devitalization”
of tumors on animal models. The
first knowledge about the immune response induced by surgical
ischemisation of a tumor by its “tiing up”, incorrecly designated as
tumor devitalizations, were presented there. Three models were discussed
– melanoblastoma in MeLiM pigs, sarcoma in inbred laboratory rat LEWIS,
and mammary gland carcinoma in bitches. Long but constructive discussion
resulted in conclusion that all the models have their pros and cons. The
presentation on melanoblastoma-infiltrating lymphocytes proved
significantly the existence of cell response following devitalization.
Regarding high specificity of this model, the results cannot be
transferred as a whole to other models. Therefore it is necessary to
search for further models which would reliably confirm or disprove the
efficiency of this therapeutic approach and allow additional
characterization of the immune response.