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3rd seminar on Progress in Veterinary Immunology
11.11.2002 13:20

September 26 – 27, 2002 a seminar named Progress in Veterinary Immunology was held in the Veterinary Research Institute, Brno. It was already the third seminar of this kind, organized in a 3-year interval by the Department of Immunology of VRI....

 

... It was held under the sponsorship of the Czech Immunological Society and the Czech Society for Analytical Cytology. Workshop offers good opportunity for making contacts and discussions about questions interesting for veterinary immunologists, it means those, for which laboratory mouse is not the dominant subject of their studies.

Ninety-six participants from various institutions took part in this-year seminar, especially from the Veterinary Research Institute, Brno; University of Veterinary and Pharmaceutical Sciences, Brno; Academy of Sciences (Institute of Microbiology, Institute of Animal Physiology and Genetics, Institute of Molecular Genetics).

The whole seminar was composed as a presentation of methodical approaches used while solving different projects. The opening brief presentation was rather a discussion topic on advantages or limitations of individual models than presentation of results. Scientific programme was divided into five sections.

In the first section – methods of molecular biology in immunology, new methodical approaches were presented in characterization of the immune response, gene rearrangement or intestinal microflora detection. Genomics, detection of VDJ rearrangement or sequencing of ribosomal RNA for characterization of microorganisms represent new trends in the methodology of immunology.

The second section – mucosal immunity – was, after an introduction on common system of mucosae and importance of microflora for activation of the immune system, oriented to two organs in whose protection mucosal immunity plays an important role – respiratory tract (pig and horse) and mammary gland (cattle). Methods of sample collection from this organs were described; the method of bronchoalveolar lavages, nasal and mammary gland lavages. The possibilities of cell population and subpopulation determination in those samples were shown using flow cytometry, and the ways of further processing of samples were discussesd for objective quantification of immunoglobulines or other soluble agents.

The third section was focused on methodical approaches in basic research in veterinary immunology. New knowledge obtained on postnatal functional maturation of the immune system of pigs and in the study of development of blood elements in pigs and dogs was presented there.

On the second day of the seminar two sections were running. Clinical immunology attracted attention of especially veterinary practitioners. Besides the general introductory papers, further contributions were devoted to individual nosological entities and possibilities of their diagnosis – immunity-mediated hemolytic anemia, systemic lupus erythematosus, rheumatoid arthritis. The possibilities of allerodiagnosis in small animals were also mentioned as well as the advantages and disadvantages of individual diagnostic methods and their asset in proper diagnosis. The question concerning the importance of secondary immunodeficiency in large production units of farm animals remained unanswered. In this field experience obtained so far in the Czech Republic is insufficient.

The last section was the most controversial, discussing the experience with “devitalizationof tumors on animal models. The first knowledge about the immune response induced by surgical ischemisation of a tumor by its “tiing up”, incorrecly designated as tumor devitalizations, were presented there. Three models were discussed – melanoblastoma in MeLiM pigs, sarcoma in inbred laboratory rat LEWIS, and mammary gland carcinoma in bitches. Long but constructive discussion resulted in conclusion that all the models have their pros and cons. The presentation on melanoblastoma-infiltrating lymphocytes proved significantly the existence of cell response following devitalization. Regarding high specificity of this model, the results cannot be transferred as a whole to other models. Therefore it is necessary to search for further models which would reliably confirm or disprove the efficiency of this therapeutic approach and allow additional characterization of the immune response.